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| Registros recuperados: 6.548 | |
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| Almeida-Val,Vera Maria Fonseca; Oliveira,Alice Reis; Silva,Maria de Nazaré Paula da; Ferreira-Nozawa,Monica Stropa; Araújo,Roziete Mendes; Val,Adalberto Luis; Nozawa,Sérgio Ricardo. |
| Adaptation or acclimation to hypoxia occurs via the modulation of physiologically relevant genes, such as erythropoietin, transferrin, vascular endothelial growth factor, phosphofructokinase and lactate dehydrogenase A. In the present study, we have cloned, sequenced and examined the modulation of the LDH-A gene after an Amazonian fish species, Astronotus crassipinis (the Oscar), was exposed to hypoxia and anoxia. In earlier studies, we have discovered that adults of this species are extremely tolerant to hypoxia and anoxia, while the juveniles are less tolerant. Exposure of juveniles to acute hypoxia and anoxia resulted in increased LDH-A gene expression in skeletal and cardiac muscles. When exposed to graded hypoxia juveniles show decreased LDH-A... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amazon; Fishes; Gene expression; RT-PCR; LDH-A; Hypoxia; Anoxia. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200025 |
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| Almeida,Wener Santos de; Belém,Francisco Ronaldo Fernandes; Bertini,Cândida Hermínia Campos de Magalhães; Pinheiro,Marcelo de Souza; Teófilo,Elizita Maria. |
| Objetivou-se com este trabalho identificar genótipos tolerantes ao estresse provocado pela solução de NaCl e pela mistura de NaCl, CaCl2 e MgCl2 na água de irrigação, nos estádios iniciais de desenvolvimento. Os níveis de condutividade elétrica do extrato de saturação foram: 0, 2,5; 5,0 e 7,5dS m-1. A tolerância do feijão-caupi foi avaliada através da redução relativa de matéria seca da parte aérea de cada genótipo. O genótipo CE-182 mostrou-se mais tolerante nos níveis 2,5; 5,0 e 7,5dS m-1 na água salinizada com solução de NaCl. Na solução de mistura dos sais, os genótipos CE-9 e CE-551 foram os mais tolerantes. De acordo com os resultados obtidos pelo agrupamento, utilizando-se o método hierárquico UPGMA, verifica-se que as combinações mais promissoras... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vigna unguiculata; Variabilidade genética; Análise multivariada. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011001100006 |
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| Almerão,Mauricio Pereira; Fagundes,Nelson Jurandi Rosa; Araújo,Paula Beatriz de; Verne,Sébastien; Grandjean,Frédéric; Bouchon,Didier; Araújo,Aldo Mellender. |
| Wolbachia are endosymbiotic bacteria that commonly infect arthropods, inducing certain phenotypes in their hosts. So far, no endemic South American species of terrestrial isopods have been investigated for Wolbachia infection. In this work, populations from two species of Balloniscus (B. sellowii and B. glaber) were studied through a diagnostic PCR assay. Fifteen new Wolbachia 16S rDNA sequences were detected. Wolbachia found in both species were generally specific to one population, and five populations hosted two different Wolbachia 16S rDNA sequences. Prevalence was higher in B. glaber than in B. sellowii, but uninfected populations could be found in both species. Wolbachia strains from B. sellowii had a higher genetic variation than those isolated from... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Wolbachia; Prevalence; Diversity; South America; Oniscidea. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600013 |
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| Al-Shammari,Maha S.; Al-Ali,Rhaya; Al-Balawi,Nader; Al-Enazi,Mansour S.; Al-Muraikhi,Ali A.; Busaleh,Fadi N.; Al-Sahwan,Ali S.; Al-Elq,Abdulmohsen; Al-Nafaie,Awatif N.; Borgio,Jesu Francis; AbdulAzeez,Sayed; Al-Ali,Amein; Acharya,Sadananda. |
| Abstract Genome-wide association studies have identified several loci associated with an increased risk for cardiovascular disease (CVD) and type 2 diabetes (T2D). Polymorphisms within the KCNQ1 (potassium voltage-gated channel, KQT-like subfamily, member 1) gene are consistently associated with T2D in a number of populations. The current study was undertaken to evaluate the association of 3 polymorphisms of KCNQ1 (rs2237892, rs151290 and rs2237895) with T2D and/or CVD. Patients diagnosed with either T2D (320 patients), CVD (250 patients) or both (60 patients) and 516 healthy controls were genotyped by TaqMan assay run on a real time PCR thermocycler. A statistically significant association was found for SNPs rs151290 (OR = 1.76; 95%CI = 1.02-3.05; p =... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: T2D; CVD; KCNQ1; Genetic association; Saudi population. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400586 |
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| Altinkut,A.; Kazan,K.; Gozukirmizi,N.. |
| The amplified fragment length polymorphism (AFLP) assay is an efficient method for the identification of molecular markers, useful in the improvement of numerous crop species. Bulked Segregant Analysis (BSA) was used to identify AFLP markers associated with water-stress tolerance in barley, as this would permit rapid selection of water-stress tolerant genotypes in breeding programs. AFLP markers linked to water-stress tolerance was identified in two DNA pools (tolerant and sensitive), which were established using selected F2 individuals resulting from a cross between water-stress-tolerant and sensitive barley parental genotypes, based on their paraquat (PQ) tolerance, leaf size, and relative water content (RWC). All these three traits were previously shown... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bulked segregant analysis; Drought; Hordeum vulgare; AFLP; Paraquat. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100013 |
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| Alvarado Beltrán, Gregorio. |
| Con la finalidad de evaluar el nivel de diversidad morfológica y genética de maíces nativos de los nichos ecológicos de Libres, Mazapiltepec y Huamantla, correspondientes a los estados de Puebla y Tlaxcala, se desarrolló la presente investigación, la cual se dividió en dos etapas. La primera comprendió una caracterización morfológica, evaluando 134 poblaciones nativas de la región de estudio, siete testigos raciales y tres testigos comerciales, en un diseño experimental látice simple 12 x 12 con dos repeticiones en tres localidades, registrándose 21 caracteres morfológicos. La segunda consistió en un análisis isoenzimático en 49 poblaciones nativas representativas y cinco testigos raciales, explorando diez sistemas... |
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Palavras-chave: Zea mays L.; Poblaciones nativas; Recursos fitogenéticos; Variabilidad genética; Landraces; Plant genetic resources; Genetic variability; Doctorado; Genética. |
| Ano: 2010 |
URL: http://hdl.handle.net/10521/182 |
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| Alvarado Beltrán, Gregorio. |
| Con la finalidad de evaluar el nivel de diversidad morfológica y genética de maíces nativos de los nichos ecológicos de Libres, Mazapiltepec y Huamantla, correspondientes a los estados de Puebla y Tlaxcala, se desarrolló la presente investigación, la cual se dividió en dos etapas. La primera comprendió una caracterización morfológica, evaluando 134 poblaciones nativas de la región de estudio, siete testigos raciales y tres testigos comerciales, en un diseño experimental látice simple 12 x 12 con dos repeticiones en tres localidades, registrándose 21 caracteres morfológicos. La segunda consistió en un análisis isoenzimático en 49 poblaciones nativas representativas y cinco testigos raciales, explorando diez sistemas... |
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Palavras-chave: Zea mays L.; Poblaciones nativas; Recursos fitogenéticos; Variabilidad genética; Landraces; Plant genetic resources; Genetic variability; Doctorado; Genética. |
| Ano: 2010 |
URL: http://hdl.handle.net/10521/182 |
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| Alvarenga,Samuel Mazzinghy; Caixeta,Eveline Teixeira; Hufnagel,Bárbara; Thiebaut,Flávia; Maciel-Zambolim,Eunize; Zambolimand,Laércio; Sakiyama,Ney Sussumu. |
| Sequences potentially associated with coffee resistance to diseases were identified by in silico analyses using the database of the Brazilian Coffee Genome Project (BCGP). Keywords corresponding to plant resistance mechanisms to pathogens identified in the literature were used as baits for data mining. Expressed sequence tags (ESTs) related to each of these keywords were identified with tools available in the BCGP bioinformatics platform. A total of 11,300 ESTs were mined. These ESTs were clustered and formed 979 EST-contigs with similarities to chitinases, kinases, cytochrome P450 and nucleotide binding site-leucine rich repeat (NBS-LRR) proteins, as well as with proteins related to disease resistance, pathogenesis, hypersensitivity response (HR) and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coffea; Data mining; ESTs; Genomics; In silico; Bioinformatics. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400031 |
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| Alvarez Alemán, A. |
| Se estudió la población del manatí antillano (Trichechus manatus) presente en la Ensenada de la Siguanea. Se colectó información acerca de los avistamientos de la especie, tamaño de los grupos, conductas, composición de la dieta y amenazas potenciales. Además se realizó la caracterización de sushábitats principales. Se utilizaron diversas metodologías que incluyeron muestreos en botes, a partir de recorridos y desde estaciones fijas, colecta e identificación de especies vegetales, colecta y análisis de material fecal y entrevistas a pescadores. Se determinó que la zona de estudio presenta un conjunto de características adecuadas para la supervivencia de la especie, como disponibilidad de alimento, temperatura del agua apropiada, fuentes de agua dulce,... |
| Tipo: Theses and Dissertations |
Palavras-chave: Habitat selection; Conservation; Aquaculture. |
| Ano: 2010 |
URL: http://hdl.handle.net/1834/5697 |
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| Alvarez,Alba; Fuentes,Jorge Luis; Puldón,Violeta; Gómez,Pedro Julio; Mora,Leonor; Duque,Miriam C.; Gallego,Gerardo; Tohme,Joe M.. |
| Microsatellite polymorphism was studied in a sample of 39 traditional rice (Oryza sativa L.) varieties and 11 improved varieties widely planted in Cuba. The study was aimed at assessing the extent of genetic variation in traditional and improved varieties and to establish their genetic relationship for breeding purposes. Heterozygosity was analyzed at each microsatellite loci and for each genotype using 10 microsatellite primer pairs. Between varieties genetic relationship was estimated. The number of alleles per microsatellite loci was 4 to 8, averaging 6.6 alleles per locus. Higher heterozygosity (H) was found in traditional varieties (H TV = 0.72) than in improved varieties (H IV = 0.42), and 68% of the total microsatellite alleles were found... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genetic diversity; Microsatellite; Rice; Traditional germplasm. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600014 |
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| Alvarez-Moya,Carlos; Reynoso Silva,Mónica; Valdez Ramírez,Carlos; Gómez Gallardo,David; León Sánchez,Rafael; Canales Aguirre,Alejandro; Feria Velasco,Alfredo. |
| There is considerable controversy with regard to the genotoxicity of glyphosate, with some reports stating that this compound is non-toxic for fish, birds and mammals. In this work, we used the comet assay to examine the genotoxicity of glyphosate isopropylamine (0.7, 7, 70 and 700 µM) in human lymphocytes, erythrocytes of Oreochromis niloticus and staminal nuclei of Tradescantia (4430) in vitro and in vivo. Cells, nuclei and fish that had and had not been exposed to 5 mM N-nitrosodiethylamine (NDEA) were used as positive and negative controls, respectively. Significant (p < 0.01) genetic damage was observed in vivo and in vitro in all cell types and organisms tested. Human lymphocytes and Tradescantia hairs showed lower genetic damage in vivo compared... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Comet assay; DNA damage; Genotoxicity; Glyphosate. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100016 |
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| Alvarez-Moya,Carlos; Reynoso Silva,Mónica; Villalobos Arámbula,Alma Rosa; Islas Sandoval,Alfonso; Castañeda Vasquez,Hugo; González Montes,Rosa María. |
| Glyphosate is noted for being non-toxic in fishes, birds and mammals (including humans). Nevertheless, the degree of genotoxicity is seriously controversial. In this work, various concentrations of a glyphosate isopropylamine salt were tested using two methods of genotoxicity assaying, viz., the pink mutation assay with Tradescantia (4430) and the comet assay with nuclei from staminal cells of the same plant. Staminal nuclei were studied in two different forms, namely nuclei from exposed plants, and nuclei exposed directly. Using the pink mutation assay, isopropylamine induced a total or partial loss of color in staminal cells, a fundamental criterion utilized in this test. Consequently, its use is not recommended when studying genotoxicity with agents... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glyphosate; DNA damage; Comet assay; Plant genotoxicity test; Tradescantia point mutation test. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100022 |
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| Registros recuperados: 6.548 | |
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